| First Author | Sakata N | Year | 2000 |
| Journal | Biochem Biophys Res Commun | Volume | 273 |
| Issue | 2 | Pages | 686-93 |
| PubMed ID | 10873665 | Mgi Jnum | J:63316 |
| Mgi Id | MGI:1860746 | Doi | 10.1006/bbrc.2000.2990 |
| Citation | Sakata N, et al. (2000) The mouse ZFH-4 protein contains four homeodomains and twenty-two zinc fingers. Biochem Biophys Res Commun 273(2):686-93 |
| abstractText | We isolated the mouse zfh-4 cDNA which is 12 kb long and capable of encoding a 3,550-amino acid protein containing four homeodomains and 22 zinc fingers including two pseudo zinc finger motifs. The mouse ZFH-4 is 51% homologous to the mouse ATBF1 and 23% to the Drosophila ZFH-2. The homeodomain and zinc finger regions are highly conserved between ZFH-4 and ATBF1 except that one zinc finger is missing in ZFH-4. Analysis of partial genomic sequences showed that the mouse zfh-4 and ATBF1 genes are similar in exon-intron organization. RT-PCR analysis of zfh-4 transcripts in adult mouse tissues showed that zfh-4 expression was low but reproducibly detectable in brain, heart, lung and muscle. In these mouse tissues, ATBF1 transcripts were poorly amplified by PCR under the conditions where zfh-4 transcripts were amplified, suggesting that the expression of zfh-4 mRNA is higher than that of ATBF1 mRNA. Other comparative analysis suggests functional similarities and dissimilarities between ZFH-4 and ATBF1. Copyright 2000 Academic Press. |
