First Author | Pagano M | Year | 1992 |
Journal | EMBO J | Volume | 11 |
Issue | 3 | Pages | 961-71 |
PubMed ID | 1312467 | Mgi Jnum | J:338151 |
Mgi Id | MGI:7510294 | Doi | 10.1002/j.1460-2075.1992.tb05135.x |
Citation | Pagano M, et al. (1992) Cyclin A is required at two points in the human cell cycle. EMBO J 11(3):961-71 |
abstractText | Cyclins play a fundamental role in regulating cell cycle events in all eukaryotic cells. The human cyclin A gene was identified as the site of integration of hepatitis B virus in a hepatocarcinoma cell line; in addition, cyclin A is associated with the E2F transcription factor in a complex which is dissociated by the E1A oncogene product. Such findings suggest that cyclin A is a target for oncogenic signals. We have now found that DNA synthesis and entry into mitosis are inhibited in human cells microinjected with anti-cyclin A antibodies at distinct times. Cyclin A binds both cdk2 and cdc2, giving two distinct cyclin A kinase activities, one appearing in S phase, the other in G2. These results suggest that cyclin A defines novel control points of the human cell cycle. |