| First Author | Abdulrazzak H | Year | 2001 |
| Journal | Mol Cell Neurosci | Volume | 17 |
| Issue | 3 | Pages | 500-13 |
| PubMed ID | 11273645 | Mgi Jnum | J:68650 |
| Mgi Id | MGI:1933023 | Doi | 10.1006/mcne.2000.0950 |
| Citation | Abdulrazzak H, et al. (2001) Structural diversity despite strong evolutionary conservation in the 5'-untranslated region of the P-type dystrophin transcript. Mol Cell Neurosci 17(3):500-13 |
| abstractText | Analysis of the 5'-flanking regions of the Purkinje (P-) dystrophin genes and mRNAs in different species revealed strong sequence conservation but functional diversity. Multiple transcription initiation sites were identified in cerebella and muscles, tissues expressing P-dystrophin. The predominant initiation site was conserved, with another muscle-specific site located upstream. Despite sequence homology, significant tissue- and species-specific structural diversity in the P-type 5'-ends exists, including alternative splicing within the 5'-untranslated region combined with alternative splicing of intron 1. One amino terminus is conserved in mammals and, to a lesser extent, in chicken. However, alternative usage of ATG codons may result in a choice of N-termini or translation of short upstream ORFs in different species. Promoter activity of a fragment upstream of the cap site was shown by transient expression in myoblasts and in vivo following intramuscular injection. It is tissue- and developmentally regulated. Analysis of promoter deletions suggests the existence of negative regulatory elements in the proximal region. Copyright 2001 Academic Press. |
