Primary Identifier | MGI:3040795 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Tpm3 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A single loxP site remained in place of the first coding exon (1b) and the donor splice junction following the excision of a floxed neo cassette by transient Cre-mediated recombination. This mutation was designed to eliminate all non-muscle type isoforms of the encoded protein. Western blot showed that protein levels in heterozygote brains were the same as that of wild-type. |