| Primary Identifier | MGI:2183146 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Tsc1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 6-8, a region of the gene conserved between mammals and fly and thought to be functionally important, were replaced with an IRES-EGFP-pA-neo cassette via homologous recombination. Splicing of exons 5-9 should result in a frameshift. Northern blot of homozygous mutant embryos detected a mutant transcript deleted for approximately 8 kb of mRNA. |
