| First Author | Carstea ED | Year | 1992 |
| Journal | Biochem Biophys Res Commun | Volume | 184 |
| Issue | 3 | Pages | 1477-83 |
| PubMed ID | 1317175 | Mgi Jnum | J:862 |
| Mgi Id | MGI:49395 | Doi | 10.1016/s0006-291x(05)80049-x |
| Citation | Carstea ED, et al. (1992) Molecular and functional characterization of the murine glucocerebrosidase gene. Biochem Biophys Res Commun 184(3):1477-83 |
| abstractText | A genomic clone of glucocerebrosidase (D-glucosyl-N-acyl-sphingosine glucohydrolase; E.C. 3.2.1.45) purified from a genomic library derived from a Balb/c mouse was analyzed by restriction mapping and nucleotide sequencing of its promoter and protein coding regions. Promoter activity was functionally assessed by ligation of a 2 kb glucocerebrosidase fragment to the protein coding segment of a bacterial neomycin resistance gene. Smaller segments of the 5' flanking sequence were then analyzed for their ability to initiate transcription of the chloramphenicol acetyltransferase reporter gene. A 319 bp Eco RI-Bgl II fragment (containing 259 bp upstream of the cDNA 5' limit) ligated to the chloramphenicol acetyltransferase open reading frame produced considerable activity. |
