| First Author | Dwyer-Nield LD | Year | 2000 |
| Journal | Am J Physiol Lung Cell Mol Physiol | Volume | 279 |
| Issue | 2 | Pages | L326-32 |
| PubMed ID | 10926556 | Mgi Jnum | J:63984 |
| Mgi Id | MGI:1888569 | Doi | 10.1152/ajplung.2000.279.2.L326 |
| Citation | Dwyer-Nield LD, et al. (2000) Quantitative trait locus mapping of genes regulating pulmonary PKC activity and PKC-alpha content. Am J Physiol Lung Cell Mol Physiol 279(2):L326-32 |
| abstractText | Strain A/J mice, which are predisposed to experimentally induced asthma and adenocarcinoma, have the lowest pulmonary protein kinase (PK) C activity and content among 22 inbred mouse strains. PKC in neonatal A/J mice is similar to that in other strains, so this difference reflects strain-dependent postnatal regulation. PKC activity is 60% higher in C57BL/6J (B6) than in A/J lungs, and the protein and mRNA concentrations of PKC-alpha, the major pulmonary PKC isozyme, are two- to threefold higher in B6 mice. These differences result from more than a single gene as assessed in F(1), F(2), and backcross progeny of B6 and A/J parents. Quantitative trait locus (QTL) analysis of 23 AxB and BxA recombinant inbred strains derived from B6 and A/J progenitors indicates a major locus regulating lung PKC-alpha content that maps near the Pkcalpha structural gene on chromosome 11 (D11MIT333; likelihood ratio statistic = 12.5) and a major locus controlling PKC activity that maps on chromosome 3 (D3MIT19; likelihood ratio statistic = 15.4). The chromosome 11 QTL responsible for low PKC-alpha content falls within QTLs for susceptibilities to lung tumorigenesis and ozone-induced toxicity. |
