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Allele : Irx3<em1Jbri> Iroquois related homeobox 3; endonuclease-mediated mutation 1, James Briscoe

Primary Identifier  MGI:7564250 Allele Type  Endonuclease-mediated
Attribute String  Epitope tag Gene  Irx3
Strain of Origin  129P2/OlaHsd-Hprt1<b-m3> Is Recombinase  false
Is Wild Type  false
molecularNote  An integration of a HA tag at the 3 ' end of the coding region of Olig2 Cas9 protein was injected directly into the blastocysts, together with guide RNAs (cgtcttaacttttcaaccat) and single-stranded DNA oligos of around 170 bp length, which carried the HA coding sequence flanked by sequences homologous to the region surrounding the natural stop codon of the corresponding gene.
  • mutations:
  • Insertion
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

2 Publication categories

Trail: Allele