First Author | Kano K | Year | 2008 |
Journal | Mol Endocrinol | Volume | 22 |
Issue | 8 | Pages | 1866-80 |
PubMed ID | 18483174 | Mgi Jnum | J:134371 |
Mgi Id | MGI:3785481 | Doi | 10.1210/me.2007-0310 |
Citation | Kano K, et al. (2008) A novel dwarfism with gonadal dysfunction due to loss-of-function allele of the collagen receptor gene, Ddr2, in the mouse. Mol Endocrinol 22(8):1866-80 |
abstractText | Smallie (slie), a spontaneous, autosomal recessive mutation causes dwarfing and infertility in mice. The purpose of this study was to determine and characterize the underlying molecular genetic basis for its phenotype. The slie locus was mapped to Chromosome 1 and fine structure mapping narrowed the slie allele within 2 Mb between genetic markers D1Mit36 and Mpz. To pinpoint the underlying mutation quantative real-time PCR was used to measure the relative expression levels for the genes residing within this region. Expression of one gene, Ddr2, which encodes discoidin domain receptor 2, was absent in slie homozygote mice. Genomic sequencing analysis detected a 150 kb deletion that extended into the Ddr2 gene transcript. Detailed phenotype analysis revealed that gonadal dysregulation underlies infertility in slie mice because all females were anovulatory and most adult males lacked spermatogenesis. The pituitary gland of pre-pubertal slie mice was smaller than in wildtype mice. The basal levels and gene expression for pituitary and hypothalamic hormones, and gene expression for hypothalamic releasing hormones were not significantly different between slie and wildtype mice. Circulating levels of IGF-1 did not differ in slie mice despite lower Igf-1 mRNA expression in the liver. After exogenous gonadotropin administration, the levels of secreted steroid hormones in both male and female adult slie mice was blunted compared to adult wildtype, but was similar to pre-pubertal wildtype mice. Taken together, our results indicate the absence of DDR2 leads to growth retardation and gonadal dysfunction due to peripheral defects in hormonal responsive pathways in slie mice. |