| Primary Identifier | MGI:7276207 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Mlh1 |
| Strain of Origin | FVB/NJ x B6(Cg)-Tyr<c-2J>/J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using an sgRNA (targeting CCACTTCCAGGGCTTTGACG) and an ssODN template (TGTGAAATGCTTCGGAGGTAGGAGGTGTGAGCGGAAGGCTTTATAGATAATGTGCTCCATAGTCCACTTCCAGGGCTTAGAGGTCGAGCCAGGCATGTCACTCTGGAAAATATATGACAC) with CRISPR/Cas9 technology, valine codon 720 (GTG) was changed to methionine (ATG) (c.2158G>A, p.V720M). This is the equivalent of the human p.V716M mutation. |
