Primary Identifier | MGI:5523980 | Allele Type | Transgenic |
Attribute String | Reporter | Gene | Tg(Thy1-GCaMP6f)GP5.11Dkim |
Strain of Origin | C57BL/6J | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The mouse promoter drives neuron-specific expression of this transgene that includes a mutagenized green fluorescent calcium indicator (GCaMP6f), woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence. GCaMP consists of a circularly permutated green fluorescent protein (cpGFP), the calcium-binding protein calmodulin (CaM), and CaM-interacting M13 peptide. This GCaMP6f variant of GCaMP incorporates A317E, T381R, S383T, and R392G mutations in CaM. Lines GP5.5 and GP5.11 were generated. |