First Author | Masumoto N | Year | 1996 |
Journal | J Cell Biol | Volume | 135 |
Issue | 6 Pt 2 | Pages | 1741-7 |
PubMed ID | 8991087 | Mgi Jnum | J:38056 |
Mgi Id | MGI:85448 | Doi | 10.1083/jcb.135.6.1741 |
Citation | Masumoto N, et al. (1996) Involvement of Rabphilin-3A in cortical granule exocytosis in mouse eggs. J Cell Biol 135(6 Pt 2):1741-7 |
abstractText | Rabphilin-3A is a putative target protein for Rab3A, a member of the small GTP-binding protein superfamily that has been suggested to play a role in regulated exocytosis in presynapses. In this study we determined the expression and the function of Rabphilin-3A in mouse eggs at fertilization. Rabphilin-3A mRNA and protein were detected by reverse transcriptase-PCR and immunoblot analysis, respectively, in metaphase II mouse eggs. Immunofluorescence analysis showed that Rabphilin-3A protein was distributed in the cortical region in eggs. Sperm induces cortical granule (CG) exocytosis via an increase in cytosolic Ca2+ at fertilization. We microinjected the NH2- or COOH-terminal fragment of recombinant Rabphilin-3A into metaphase II eggs. Neither treatments altered the sperm-induced cytosolic Ca2+ increase, but both inhibited CG exocytosis in a dose-dependent manner. The NH2-terminal fragment was more effective than the COOH-terminal fragment. Full-length Rabphilin-3A did not affect CG exocytosis, but it attenuated the inhibition of CG exocytosis by the NH2-terminal fragment. These results show that Rabphilin-3A is involved in Ca(2+)-dependent CG exocytosis at fertilization in mouse eggs. |