| First Author | Crooks ME | Year | 1994 |
| Journal | Immunity | Volume | 1 |
| Issue | 4 | Pages | 277-85 |
| PubMed ID | 7889415 | Mgi Jnum | J:112551 |
| Mgi Id | MGI:3662700 | Doi | 10.1016/1074-7613(94)90079-5 |
| Citation | Crooks ME, et al. (1994) Disruption of T lymphocyte positive and negative selection in mice lacking the CD8 beta chain. Immunity 1(4):277-85 |
| abstractText | The CD4 and CD8 coreceptors have been shown to play significant roles in the differentiation and activation of helper and cytotoxic T lymphocytes (CTLs), respectively. Coordinate binding of coreceptor and T cell receptor (TCR) to the same major histocompatibility complex (MHC) molecule and coreceptor interaction with the tyrosine kinase p56lck are required for effective signaling. Whereas CD4 is a monomer, CD8 consists of either alpha alpha homodimers or alpha beta heterodimers. Signaling properties of CD8 have been ascribed to the alpha chain, which binds to both the MHC class I and to p56lck, respectively. To study CD8 beta specifically, we have generated mice defective in its expression. We observe a significant reduction in the numbers of CD8+ T cells, but these cells have normal CTL activity. By breeding CD8 beta null mice with animals expressing a class I-specific TCR transgene, we show that CD8 beta plays a significant role in both positive and negative selection of developing thymocytes. |
