| First Author | Lee TH | Year | 1999 |
| Journal | Gene | Volume | 234 |
| Issue | 2 | Pages | 337-44 |
| PubMed ID | 10395907 | Mgi Jnum | J:56276 |
| Mgi Id | MGI:1340729 | Doi | 10.1016/s0378-1119(99)00190-0 |
| Citation | Lee TH, et al. (1999) Characterization of the murine gene encoding 1-Cys peroxiredoxin and identification of highly homologous genes. Gene 234(2):337-44 |
| abstractText | A new type of peroxiredoxin, named 1-Cys peroxiredoxin (1- Cys Prx), reduces hydrogen peroxide with the use of electrons from unidentified electron donor(s). We have isolated the mouse gene encoding 1-Cys Prx (CP-3) and shown that it is comprised of five exons and four introns. Analysis of 5' flanking regions revealed binding sequences of several putative transcription factors such as Sp1. Pit- la, c-Jun, c-Myc and YY1. It is noticeable that several potential Spl binding sites assigned the -60 through -96 bp from putative transcription initiation site, The gel shift assays showed that Spl and Pit-1a bind specifically to each binding site in I-Cys Prx promoter. We also isolated two highly related genes such as CP-2 and CP-5. These genes are encoded by single exons, and show 85% of nucleotide sequence homology with the CP-3. The structural features of these genes suggest that they might be intronless genes derived from the CP-3 by the mechanism involving retrotransposition. In addition, our data suggest that they are inserted to a specific site of the mouse L1 repetitive element. The 1-Cys Prx was actively transcribed in a variety of adult tissues as well as in the developing embryos. These results suggest that only the 1-Cys Prx gene might be relevant for studying the function of the 1-Cys Prx in the murine system. (C) 1999 Elsevier Science B.V. All rights reserved. |
