| First Author | Le YZ | Year | 2006 |
| Journal | Adv Exp Med Biol | Volume | 572 |
| Pages | 173-8 | PubMed ID | 17249572 |
| Mgi Jnum | J:220520 | Mgi Id | MGI:5634936 |
| Doi | 10.1007/0-387-32442-9_26 | Citation | Le YZ, et al. (2006) Conditional gene knockout system in cone photoreceptors. Adv Exp Med Biol 572:173-8 |
| abstractText | To study function of widely expressed essential genes, we established a conditional knockout system for cone photoreceptor cells. Our goal is to generate a useful genetic system that can be utilized to disrupt gene function efficiently in cone photoreceptor cells. Functional assay using a Cre-activatable lacZ reporter gene suggested that HRGP-cre mice had widely expressed functional Cre in cone photoreceptors. Since nearly all cone photoreceptor cells express Cre in our transgenic mice, they will be efficient in carrying out Cre-mediated gene activation and inactivation in both M- and S-cone photoreceptors. In addition, we have not observed any apparent ectopic expression in HRGP-cre mice (Le et al., 2004); thus, conditional knockout of essential genes with these mice is not likely to cause any detrimental effect in non-ocular tissues. |
