Primary Identifier | MGI:5441750 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Peli3 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site and puro cassette were inserted upstream of exon 3 and a loxP site was inserted downstream of exon 3 via homologous recombination. Cre mediated recombination removed the puro cassette and exon 3. Semi-quantitative and real-time RT-PCR analysis confirmed the absence of mRNA expression in MEFs from homozygous embryos. |