Primary Identifier | MGI:7430731 | Allele Type | Endonuclease-mediated |
Attribute String | Conditional ready, Inserted expressed sequence | Gene | Gt(ROSA)26Sor |
Strain of Origin | C57BL/6NTac | Is Recombinase | false |
Is Wild Type | false |
molecularNote | A targeting vector inserted a loxp flanked stop cassette in intron1 of the Rosa26 locus followed immediately by a wild-type murine Btg1 cDNA-T2A-Luciferase sequence. The allele was generated by introducing a single-nucleotide substitution to revert the Q36H mutation of the cDNA sequence in Gt(ROSA)26Sortm1(Btg1*)Meln to a wild-type sequence by CRISPR-Cas9-mediated genome targeting. |