| First Author | Jones DT | Year | 1987 |
| Journal | J Biol Chem | Volume | 262 |
| Issue | 29 | Pages | 14241-9 |
| PubMed ID | 2820999 | Mgi Jnum | J:8848 |
| Mgi Id | MGI:57313 | Doi | 10.1016/s0021-9258(18)47929-x |
| Citation | Jones DT, et al. (1987) Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium. J Biol Chem 262(29):14241-9 |
| abstractText | Biochemical studies in vertebrate olfactory tissue indicate that certain odorants stimulate adenylyl cyclase in a GTP-dependent manner. Additionally, immunochemical and toxin-labeling studies demonstrate the presence of several GTP-binding protein (G-protein) species in vertebrate olfactory epithelium. To identify the G-protein(s) responsible for olfactory signal transduction, we screened a rat olfactory cDNA library with an oligonucleotide probe and isolated 32 recombinant clones encoding five distinct types of G-protein alpha subunits. The majority of the clones encoded G alpha s, while the remaining clones encoded G alpha o, G alpha i1, G alpha i2, and a novel species, G alpha i3. Messenger RNA corresponding to each G alpha was detectable in all tissues examined; however, the levels for a given G alpha varied in a tissue-specific manner. In olfactory tissue, G alpha s was the most abundant of these messages and in combination with the biochemical studies suggests that G alpha s is the G-protein component of the olfactory signal transduction cascade. |
