| First Author | Giannoni F | Year | 2005 |
| Journal | J Virol | Volume | 79 |
| Issue | 11 | Pages | 6808-13 |
| PubMed ID | 15890920 | Mgi Jnum | J:98390 |
| Mgi Id | MGI:3578090 | Doi | 10.1128/JVI.79.11.6808-6813.2005 |
| Citation | Giannoni F, et al. (2005) Protein kinase C theta is not essential for T-cell-mediated clearance of murine gammaherpesvirus 68. J Virol 79(11):6808-13 |
| abstractText | Murine gammaherpesvirus 68 (MHV-68) is a naturally occurring rodent pathogen with significant homology to human pathogens Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus. T cells are essential for primary clearance of MHV-68 and survival of mice following intranasal infection. Previous reports have suggested that protein kinase C theta (PKCtheta) is essential for T-cell activation and cytokine production in vitro. To determine the role of this molecule in vivo during the immune response to a viral infection, PKCtheta-/- mice were infected with MHV-68. Despite the essential role of T cells in viral clearance, PKCtheta-/- mice survived infection, cleared lytic virus, and maintained effective long-term control of latency. CD8 T-cell expansion, trafficking to the lung, and cytotoxic activity were similar in PKCtheta+/+ and PKCtheta-/- mice, whereas antiviral antibody and T-helper cell cytokine production were significantly lower in PKCtheta-/- mice than in PKCtheta+/+ mice. These studies demonstrate a differential requirement for PKCtheta in the immune response to MHV-68 and show that PKCtheta is not essential for the T-cell activation events leading to viral clearance. |
