| First Author | Ebensperger C | Year | 1996 |
| Journal | Scand J Immunol | Volume | 44 |
| Issue | 6 | Pages | 599-606 |
| PubMed ID | 8972742 | Mgi Jnum | J:37979 |
| Mgi Id | MGI:85372 | Doi | 10.1046/j.1365-3083.1996.d01-353.x |
| Citation | Ebensperger C, et al. (1996) Genomic organization and promoter analysis of the gene ifngr2 encoding the second chain of the mouse interferon-gamma receptor. Scand J Immunol 44(6):599-606 |
| abstractText | A clone containing the gene ifngr2 for the second chain (IFN-gamma R2) of the mouse interferon gamma receptor complex was isolated from a cosmid library made of 129/Sv mouse genomic DNA. Sequence analysis revealed that the second chain is encoded by 7 exons. The complete gene spans about 17 kb of the genomic DNA. In the 5'-flanking region several transcription initiation sites between 27 and 136 nucleotides upstream from the translation initiation codon were mapped. This region has a high GC content, but no TATA or CAAT box. Potential binding sites were found for transcription factors Sp1, AP-2, NF1, EGR and NF kappa B. Promoter activity was assayed with a series of constructs with firefly luciferase as a reporter gene, under the control of the promoter fragments of various lengths. This region showed promoter activity in transiently transfected Chinese hamster ovary cells. |
