| First Author | Lindley LE | Year | 2013 |
| Journal | Genesis | Volume | 51 |
| Issue | 7 | Pages | 491-7 |
| PubMed ID | 23495064 | Mgi Jnum | J:199325 |
| Mgi Id | MGI:5502275 | Doi | 10.1002/dvg.22390 |
| Citation | Lindley LE, et al. (2013) Generation of mice with a conditional Lbh null allele. Genesis 51(7):491-7 |
| abstractText | Limb bud and heart (LBH) is a developmentally expressed, tissue-specific transcription cofactor in vertebrates that acts in the WNT signaling pathway, a genetic program critical for embryogenesis and adult tissue homeostasis. Aberrant gain-of-function of LBH is implicated in both human congenital disease and cancer. The normal physiological function of LBH has remained elusive owing to a lack of genetic loss-of-function models. Here, we have generated mice with a conditional null allele of Lbh by flanking exon 2 with loxP sites (Lbh(flox) ). Homozygous Lbh(flox) and Lbh(loxP) mice, in which the Neo cassette was removed through FLPe-mediated recombination, were viable and fertile, indicating that these conditional Lbh alleles are fully functional. Lbh(loxP) mice were then crossed with a Rosa26-Cre line, resulting in ubiquitous deletion of exon 2 and abolishment of LBH protein expression. Mice homozygous for the Lbh null allele (Lbh(Delta) (2) ) displayed normal embryonic development and postnatal growth with morphologies indistinguishable from wild-type littermates. However, mammary gland development, which occurs primarily after birth, was perturbed. Thus, the conditional Lbh allele will be a valuable tool to uncover the currently unknown tissue-specific roles of LBH in postnatal development and disease. genesis 51:491-497. (c) 2013 Wiley Periodicals, Inc. |
