Primary Identifier | MGI:5614756 | Allele Type | Targeted |
Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting construct inserted into the locus a CAG promoter, floxed membrane-targeted tandem-dimer Tomato (mT) reporter gene, an improved sensor cGMP indicator (cGi500; containing an FRET-activated, cGMP-sensitive ECFP/EYFP fusion) and an FRT-flanked neomycin resistance cassette. Cre-mediated recombination can remove the Tomato reporter gene to allow the expression of the cGMP indicator in a time- and cell type-specific manner. The cGMP indicator has an EC50 of 500 nmol/L. |