Primary Identifier | MGI:6416530 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Rere |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 9.6-kb fragment containing the second coding exon of Rere was retrieved into a pBluescript-derived plasmid containing 500-bp homology arms from BAC clone bMQ386d15 selected from the M37- 129AB22 BAC library. A neomycin resistance cassette flanked by two loxP sites was then inserted 5' to the second coding exon of Rere. A neomycin resistance cassette flanked by an frt site, and an frt and loxP site marked with a BglII restriction site was then inserted 3' to the second coding exon to generate the final targeting vector. |