| First Author | Park WJ | Year | 2008 |
| Journal | Neurosci Lett | Volume | 444 |
| Issue | 3 | Pages | 240-4 |
| PubMed ID | 18760330 | Mgi Jnum | J:141196 |
| Mgi Id | MGI:3817394 | Doi | 10.1016/j.neulet.2008.08.044 |
| Citation | Park WJ, et al. (2008) Leucine-rich glioma inactivated 3 associates with syntaxin 1. Neurosci Lett 444(3):240-4 |
| abstractText | Leucine-rich glioma inactivated 3 (LGI3) is a member of LGI/epitempin (EPTP) family. The biological function of LGI3 and its association with disease are not known. We previously reported that mouse LGI3 was highly expressed in brain in a developmentally and transcriptionally regulated manner. In this study, we identified syntaxin 1, a SNARE component in exocytosis, as a candidate functional target of LGI3. Western blot analysis of mouse brain extract with LGI3 antibodies detected multiple protein forms (75-, 60-, 35- and 25-kDa). Proteomic analysis, pull-down and coimmunoprecipitation experiments identified syntaxin 1 as an LGI3-associated protein. LGI3 colocalized with syntaxin 1 in processes of cortical neurons with punctate synaptic pattern and was enriched in synaptosomal fraction. Coimmunoprecipitation showed that LGI3-syntaxin 1 complex did not contain other SNARE components, SNAP25 and VAMP2. Recombinant LGI3 attenuated Ca(2+)-evoked glutamate release from digitonin-permeabilized synaptosomes and transfection of PC12 cells with LGI3 decreased K(+)-induced secretion of human growth hormone. Thus, LGI3 may play a regulatory role in neuronal exocytosis via its interaction with syntaxin 1. |
