| First Author | Ozaki N | Year | 2012 |
| Journal | Genes Cells | Volume | 17 |
| Issue | 2 | Pages | 109-21 |
| PubMed ID | 22244086 | Mgi Jnum | J:203608 |
| Mgi Id | MGI:5527521 | Doi | 10.1111/j.1365-2443.2011.01574.x |
| Citation | Ozaki N, et al. (2012) Regulation of basic helix-loop-helix transcription factors Dec1 and Dec2 by RORalpha and their roles in adipogenesis. Genes Cells 17(2):109-21 |
| abstractText | DEC1 and DEC2, members of the basic helix-loop-helix superfamily, are involved in various biological phenomena including clock systems, cell differentiation and metabolism. In clock systems, Dec1 and Dec2 expression are up-regulated by the CLOCK:BMAL1 heterodimer via E-box (CACGTG), exhibiting a circadian rhythm in the suprachiasmatic nucleus (SCN), the central circadian pacemaker and other peripheral tissues. In this study, using assays of luciferase reporters, electrophoretic mobility shift and chromatin immunoprecipitation, we identified novel nuclear receptor response elements, ROR response elements (RORE), in Dec1 and Dec2 promoters. These ROREs responded to the transcriptional activator RORalpha, but not to the repressor REVERBalpha, although the Bmal1 promoter responded to both RORalpha and REVERBalpha. Therefore, RORalpha, but not REVERBalpha, is involved in the regulation of Dec1 and Dec2 expression without significantly affecting their rhythmicity. Since RORalpha, DEC1 and DEC2 reportedly suppressed adipogenic differentiation, we examined expression of Roralpha, Dec1, Dec2 and other clock-controlled genes in differentiating 3T3-L1 adipocytes. The results suggested that RORalpha suppresses adipogenic differentiation at a later stage of differentiation by RORE-mediated stimulation of Dec1 and Dec2 expression. |
