| First Author | Cybulsky MI | Year | 1993 |
| Journal | Genomics | Volume | 18 |
| Issue | 2 | Pages | 387-91 |
| PubMed ID | 7507076 | Mgi Jnum | J:15607 |
| Mgi Id | MGI:63724 | Doi | 10.1006/geno.1993.1480 |
| Citation | Cybulsky MI, et al. (1993) Structure of the murine VCAM1 gene. Genomics 18(2):387-91 |
| abstractText | The architecture of the murine VCAM1 gene, encoding vascular cell adhesion molecule-1, was determined. its 10 exons span approximately 20 kb. Exon 1 encodes the 5' untranslated region and the signal peptide; exons 2-4 and 6-9, the C2 or H-type immunoglobulin domains; and exon 10, the transmembrane and cytoplasmic domains followed by the entire 3' untranslated region. All immunoglobulin-like domains are encoded by separate exons, and exon splice junctions occur after the first nucleotide of amino acid codons (type 1). Exon 5 encodes a novel domain unique to murine VCAM-1 and inclusion of this exon by alternative splicing results in a truncated three-immunoglobulin-like domain form, which is bound to the cell membrane by a phosphatidylinositol linkage at its carboxy terminus (encoded by exon 5). The murine VCAM1 core promoter contains a high degree of homology to the human, including conserved consensus binding sites for NF-kappa B, the Ets class, and the GATA family of transcription factors, suggesting that the murine and human VCAM1 genes may be under similar transcriptional control. |
