| First Author | Bharathavikru R | Year | 2017 |
| Journal | Genes Dev | Volume | 31 |
| Issue | 4 | Pages | 347-352 |
| PubMed ID | 28289143 | Mgi Jnum | J:312420 |
| Mgi Id | MGI:6783373 | Doi | 10.1101/gad.291500.116 |
| Citation | Bharathavikru R, et al. (2017) Transcription factor Wilms' tumor 1 regulates developmental RNAs through 3' UTR interaction. Genes Dev 31(4):347-352 |
| abstractText | Wilms' tumor 1 (WT1) is essential for the development and homeostasis of multiple mesodermal tissues. Despite evidence for post-transcriptional roles, no endogenous WT1 target RNAs exist. Using RNA immunoprecipitation and UV cross-linking, we show that WT1 binds preferentially to 3' untranslated regions (UTRs) of developmental targets. These target mRNAs are down-regulated upon WT1 depletion in cell culture and developing kidney mesenchyme. Wt1 deletion leads to rapid turnover of specific mRNAs. WT1 regulates reporter gene expression through interaction with 3' UTR-binding sites. Combining experimental and computational analyses, we propose that WT1 influences key developmental and disease processes in part through regulating mRNA turnover. |
