Primary Identifier | MGI:5140189 | Allele Type | Targeted |
Attribute String | Modified regulatory region | Gene | Rr27 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Site-directed mutagenesis destroyed the eight CpG islands outside of the CTCF sites. A floxed neomycin resistance gen cassette inserted downstream of the modified sequence was removed by cre-mediated recombination removed. The CpG content of the imprinting control region (ICR) is reduced 16% without changing the size of the ICR or disrupting the CTCF sites. |