Primary Identifier | MGI:5317867 | Allele Type | Targeted |
Attribute String | Hypomorph, Modified regulatory region | Gene | Pax6 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site and an Sph I site were inserted upstream of the distal enhancer element and the 450 bp proximal enhancer element was replaced with an FRT flanked neo cassette and a loxP site. Recombinase mediated recombination removed the neo cassette and a 3.1 kb region including the distal and the ectodermal enhancer elements. RT-PCR analysis indicates reduced pancreatic expression in homozygous mice at E15.5. |