| First Author | Hiemstra IH | Year | 2014 |
| Journal | Immunology | Volume | 142 |
| Issue | 2 | Pages | 269-78 |
| PubMed ID | 24883436 | Mgi Jnum | J:212389 |
| Mgi Id | MGI:5581346 | Doi | 10.1111/imm.12251 |
| Citation | Hiemstra IH, et al. (2014) The identification and developmental requirements of colonic CD169(+) macrophages. Immunology 142(2):269-78 |
| abstractText | CD169-positive macrophages in the marginal zone of the spleen and subcapsular sinus of lymph nodes play an important role as gatekeepers, strategically located to capture pathogens. Here we identified a population of CD169-positive macrophages in the colon and investigated which factors influenced their development. Murine colonic CD115+ F4/80(lo) CD11c(lo) macrophages expressing CD169 were present in the lamina propria, mainly surrounding the crypts. In spite of the high levels of bacterial flora in the colon and the importance of Toll-like receptor signalling in mucosal homeostasis, the presence of CD169+ macrophages was not affected in mice that were deficient in MyD88-mediated Toll-like receptor signalling and in mice in which the bacterial flora was eradicated. Whereas the development of splenic CD169+ macrophages was dependent on lymphotoxin alpha, colonic CD169+ macrophages were present in normal numbers in lymphotoxin alpha-deficient mice. In contrast, reduced numbers of CD169+ macrophages were found in the colon of mice deficient in vitamin A, whereas CD169+ macrophages in the spleen were unaffected. In conclusion, we identified a new macrophage subset in the lamina propria of the colon characterized by the expression of CD169. Its differentiation, unlike CD169+ macrophages in lymphoid organs, is independent of lymphotoxin alpha signalling, but requires vitamin A. |
