| First Author | Beccari T | Year | 1992 |
| Journal | Biochem J | Volume | 285 ( Pt 2) |
| Pages | 593-6 | PubMed ID | 1379046 |
| Mgi Jnum | J:1472 | Mgi Id | MGI:49999 |
| Doi | 10.1042/bj2850593 | Citation | Beccari T, et al. (1992) Cloning and sequence analysis of a cDNA encoding the alpha-subunit of mouse beta-N-acetylhexosaminidase and comparison with the human enzyme. Biochem J 285(Pt 2):593-6 |
| abstractText | cDNAs encoding the mouse beta-N-acetylhexosaminidase alpha-subunit were isolated from a mouse testis library. The longest of these (1.7 kb) was sequenced and showed 83% similarity with the human alpha-subunit cDNA sequence. The 5' end of the coding sequence was obtained from a genomic DNA clone. Alignment of the human and mouse sequences showed that all three putative N-glycosylation sites are conserved, but that the mouse alpha-subunit has an additional site towards the C-terminus. All eight cysteines in the human sequence are conserved in the mouse. There are an additional two cysteines in the mouse alpha-subunit signal peptide. All amino acids affected in Tay-Sachs-disease mutations are conserved in the mouse. |
