| First Author | Olds-Clarke P | Year | 1992 |
| Journal | Biol Reprod | Volume | 47 |
| Issue | 4 | Pages | 621-8 |
| PubMed ID | 1391349 | Mgi Jnum | J:2574 |
| Mgi Id | MGI:51096 | Doi | 10.1095/biolreprod47.4.621 |
| Citation | Olds-Clarke P, et al. (1992) Impaired transport and fertilization in vivo of calcium-treated spermatozoa from +/+ or congenic tw32/+ mice. Biol Reprod 47(4):621-8 |
| abstractText | To determine whether calcium alters processes important for fertilization in vivo, mouse (+/+) spermatozoa were incubated in medium with 1.0-1.7 mM calcium prior to artificial insemination (AI) into the cervix of hormonally primed females. Spermatozoa from congenic tw32/+ mice were also tested because their flagella are hypersensitive to calcium. As a control, spermatozoa were incubated in calcium-deficient medium prior to AI. Spermatozoa from mice of both genotypes incubated in calcium-containing medium fertilized significantly fewer eggs after AI than did spermatozoa incubated in calcium-deficient medium. In addition, calcium-treated spermatozoa from tw32/+ mice fertilized significantly fewer eggs than calcium-treated +/+ spermatozoa. Pretreatment with calcium also reduced the number of spermatozoa in the oviducts 0.5-4.5 h after AI, and the oviducts of females inseminated with calcium-treated spermatozoa from tw32/+ mice contained significantly fewer spermatozoa than those of females inseminated with calcium-treated +/+ spermatozoa. These results suggest that preincubation in millimolar levels of calcium changes the physiology of epididymal spermatozoa in such a way as to impair sperm transport to the oviduct and fertilization in vivo. |
