| Type | MGI:General | Description | after skine exposure to DNFB, lymph nodes examined 5 hours later after incubation with DNBS show 97% less T cell proliferation compared to wild-type lymph node T cells lymph node T cells proliferate comparably in vitro to wild-type controls in response to polyclonal activation with PMA + Ionomycin, concanavalin A or CD3 antibodies when dendritic cells are conjugated with DNBS and injected intravenously or subcutaneously, proliferation of T cells is substantial in splenic T cell cultures but proliferation in lymph node cultures is similar to background; in wild-type mice, T cell proliferation in splenic cultures occurs, but to a lesser extent than in lymph node cultures; results indicate that once response dependence on peripheral lymph nodes is removed, delayed type hypersensitization immune responses are intact |
