Primary Identifier | MGI:2179990 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Ckm |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 0.9 kb region encompassing exon 2 and part of introns 1 and 2 was replaced with a hygroB resistance cassette via homologous recombination. Northern blot using an exon 2 probe demonstrated lack of gene transcripts in skeletal muscle and staining for enzyme activity was negative in homogenates of skeletal and cardiac muscle from homozygous mutant animals. |