| First Author | Sakaguchi N | Year | 1986 |
| Journal | EMBO J | Volume | 5 |
| Issue | 9 | Pages | 2139-47 |
| PubMed ID | 2430794 | Mgi Jnum | J:8478 |
| Mgi Id | MGI:56944 | Doi | 10.1002/j.1460-2075.1986.tb04477.x |
| Citation | Sakaguchi N, et al. (1986) Isolation of a cDNA copy of an RNA species expressed in murine pre-B cells. EMBO J 5(9):2139-47 |
| abstractText | A part of a gene, pZ 183, was isolated from a cDNA library constructed from poly(A)-containing RNA of the chemically induced murine pre-B lymphoma 70Z/3. This DNA sequence was found to be present in one of 5,000 cDNA clones in the unsubtracted library, and in one of 350 cDNA clones in a library in which sequences common to 70Z/3 and to a T cell hybridoma had been subtracted. This indicates that the corresponding gene belongs to the genes which are expressed with low to medium abundance in the pre-B cell line 70Z/3. The gene was found to be expressed in pre-B lymphomas, but not in B lymphomas, myelomas, T cell lymphomas, macrophage tumors or a fibroblast line. By in situ hybridization it was detected in 2-4% of fetal liver cells between day 15 and 16 of gestation, and in 1% of adult bone marrow cells. It was not detected in thymus cells, nor in mature T cells, spleen, adult liver, kidney, heart, lung or brain. The size of the transcript found in all pre-B cell lymphomas is 1.2 kb in length. The pZ 183 DNA sequence would appear to be a useful B-lineage specific marker which identifies pre-B cells at various stages before the mature B cell. |
