| First Author | Laurie GW | Year | 1995 |
| Journal | Biochem Biophys Res Commun | Volume | 217 |
| Issue | 1 | Pages | 10-5 |
| PubMed ID | 8526896 | Mgi Jnum | J:30593 |
| Mgi Id | MGI:78092 | Doi | 10.1006/bbrc.1995.2738 |
| Citation | Laurie GW, et al. (1995) Immunological and partial sequence identity of mouse BM180 with wheat alpha-gliadin. Biochem Biophys Res Commun 217(1):10-5 |
| abstractText | BM180, a novel 180-kDa basement membrane protein enriched in guanidine-HCl extracts of lacrimal and parotid exocrine secretory glands, was immunopurified using the secretion inhibitory monoclonal antibody 3E12. The N-terminal amino acid sequence was found to be VRVPVPQLQPQNP. An identical sequence comprises the N-terminus of the wheat storage protein alpha-gliadin. The presence of a gliadin-like protein in basement membranes was confirmed using a monoclonal and several polyclonal anti-gliadin antibodies, the former of which detected a 180-kDa protein in basement membrane blots. A full-length alpha-gliadin cDNA was found to hybridize at high stringency with mouse and human genomic DNA; and in lacrimal gland Northern blots with a 2.3-kb message. Since BM180 appears to be required for stimulus-secretion coupling by lacrimal acinar cells, circulating anti-alpha-gliadin antibodies associated with Sjogren's syndrome ('Dry Eye') and more commonly in Coeliac disease, may be secretion inhibitory. |
