| First Author | Ishibashi J | Year | 2012 |
| Journal | Mol Cell Biol | Volume | 32 |
| Issue | 12 | Pages | 2289-99 |
| PubMed ID | 22473998 | Mgi Jnum | J:185720 |
| Mgi Id | MGI:5429783 | Doi | 10.1128/MCB.06529-11 |
| Citation | Ishibashi J, et al. (2012) An Evi1-C/EBPbeta complex controls peroxisome proliferator-activated receptor gamma2 gene expression to initiate white fat cell differentiation. Mol Cell Biol 32(12):2289-99 |
| abstractText | Fibroblastic preadipocyte cells are recruited to differentiate into new adipocytes during the formation and hyperplastic growth of white adipose tissue. Peroxisome proliferator-activated receptor gamma (PPARgamma), the master regulator of adipogenesis, is expressed at low levels in preadipocytes, and its levels increase dramatically and rapidly during the differentiation process. However, the mechanisms controlling the dynamic and selective expression of PPARgamma in the adipocyte lineage remain largely unknown. We show here that the zinc finger protein Evi1 increases in preadipocytes at the onset of differentiation prior to increases in PPARgamma levels. Evi1 expression converts nonadipogenic cells into adipocytes via an increase in the predifferentiation levels of PPARgamma2, the adipose-selective isoform of PPARgamma. Conversely, loss of Evi1 in preadipocytes blocks the induction of PPARgamma2 and suppresses adipocyte differentiation. Evi1 binds with C/EBPbeta to regulatory sites in the Ppargamma locus at early stages of adipocyte differentiation, coincident with the induction of Ppargamma2 expression. These results indicate that Evi1 is a key regulator of adipogenic competency. |
