Primary Identifier | MGI:7468701 | Allele Type | Endonuclease-mediated |
Attribute String | Humanized sequence | Gene | Chrna5 |
Inheritance Mode | Not Specified | Strain of Origin | C57BL/6J |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Aspartic acid codon 398 (GAT) in exon 6 was changed to asparagine (AAT) (p.D398N) using an sgRNA (targeting CCGCGCYCGATTGCATTCGCTAC) and an ssODN template with CRISPR/Cas9 technology. The mutation is the equivalent of human SNP rs16969968 (p.D398N) associated with nicotine and opiate dependence. |