Primary Identifier | MGI:7545158 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Ankdd1a |
Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
Is Recombinase | false | Is Wild Type | false |
Project Collection | IMPC |
molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CACTAACCTGCCCTACCCCA and ATACAAGTGATACAGAACCT, which resulted in a 320 bp deletion beginning at Chromosome 9 position 65,503,339 bp and ending after 65,503,658 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000246940 (exon 12) and 172 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 367 and early truncation 76 amino acids later. |