| First Author | Chen L | Year | 2011 |
| Journal | J Cell Sci | Volume | 124 |
| Issue | Pt 14 | Pages | 2478-87 |
| PubMed ID | 21693581 | Mgi Jnum | J:183044 |
| Mgi Id | MGI:5317381 | Doi | 10.1242/jcs.078170 |
| Citation | Chen L, et al. (2011) Protein 4.1R regulates cell adhesion, spreading, migration and motility of mouse keratinocytes by modulating surface expression of beta1 integrin. J Cell Sci 124(Pt 14):2478-87 |
| abstractText | Protein 4.1R is a membrane-cytoskeleton adaptor protein that has diverse roles in controlling the cell surface expression and/or function of transmembrane proteins, and in organizing F-actin. 4.1R is expressed in keratinocytes, but its role in these cells has not been explored. Here, we have investigated the role of 4.1R in skin using 4.1R(-/-) mice. Cell adhesion, spreading, migration and motility were significantly impaired in 4.1R(-/-) keratinocytes, and 4.1R(-/-) mice exhibited defective epidermal wound healing. Cultured 4.1R(-/-) keratinocytes on fibronectin failed to form actin stress fibres and focal adhesions. Furthermore, in the absence of 4.1R, the surface expression, and consequently the activity of beta1 integrin were reduced. These data enabled the identification of a functional role for protein 4.1R in keratinocytes by modulating the surface expression of beta1 integrin, possibly through a direct association between 4.1R and beta1 integrin. |
