| First Author | Hayakawa K | Year | 1983 |
| Journal | J Exp Med | Volume | 157 |
| Issue | 1 | Pages | 202-18 |
| PubMed ID | 6600267 | Mgi Jnum | J:6923 |
| Mgi Id | MGI:55395 | Doi | 10.1084/jem.157.1.202 |
| Citation | Hayakawa K, et al. (1983) The Ly-1 B cell subpopulation in normal immunodefective, and autoimmune mice. J Exp Med 157(1):202-18 |
| abstractText | A small subpopulation of normal murine splenic B cells carrying all of the classic B cells markers (IgM, IgD, Ia, and ThB) also carries Ly-1, one of the major T cell surface molecules. This Ly-1 B subpopulation (identified and characterized by multiparameter FACS analyses) consists of relatively large, high IgM/low-IgD/low-Ly-1 lymphocytes that represent approximately 2% of the spleen cells in normal animals and, generally, 5-10% of spleen cells in NZB mice. Ly-1 B are clearly detectable in all normal mouse strains tested as well as NZB, CBA/N, other X-id mice and nude (nu/nu) mice. They are found primarily in the spleen; are either absent or very poorly represented in lymph node, bone marrow, and thymus; appear early during ontogeny, and comprise about a third of the small number of lymphocytes present in 5-d-old mice. NZB and (NZB x NZW)F1 mice have more Ly-1 B than all other strains and, furthermore, have a unique Ly-1 B population that secretes IgM when cultured under usual conditions in the absence of added antigen. The IgM secretion by these Ly-1 B cells accounts for the previously reported spontaneous IgM secretion by NZB spleen cells in culture. Studies with FACS-sorted cells show that the presence of Ly-1 on these IgM-secreting cells distinguishes them from the (Ly-1 negative) IgM-secreting direct plaque-forming cells generated in NZB mice after stimulation with sheep erythrocytes. |
