| First Author | Wang BB | Year | 2006 |
| Journal | Biochem Biophys Res Commun | Volume | 347 |
| Issue | 4 | Pages | 1129-37 |
| PubMed ID | 16870143 | Mgi Jnum | J:111640 |
| Mgi Id | MGI:3654606 | Doi | 10.1016/j.bbrc.2006.07.020 |
| Citation | Wang BB, et al. (2006) Inducible and reversible suppression of Npm1 gene expression using stably integrated small interfering RNA vector in mouse embryonic stem cells. Biochem Biophys Res Commun 347(4):1129-37 |
| abstractText | The tetracycline (Tc)-inducible small interference RNA (siRNA) is a powerful tool for studying gene function in mammalian cells. However, the system is infrequently utilized in embryonic stem (ES) cells. Here, we present the first application of the Tc-inducible, stably integrated plasmid-based siRNA system in mouse ES cells to down-regulate expression of Npm1, an essential gene for embryonic development. The physiological role of Npm1 in ES cells has not been defined. Our data show that the knock-down of Npm1 expression by this siRNA system was not only highly efficient, but also Tc- dose- and induction time-dependent. Particularly, the down-regulation of Npm1 expression was reversible. Importantly, suppression of Npm1 expression in ES cells resulted in reduced cell proliferation. Taken together, this system allows for studying gene function in a highly controlled manner, otherwise difficult to achieve in ES cells. Moreover, our results demonstrate that Npm1 is essential for ES cell proliferation. |
