| First Author | Murphy S | Year | 2017 |
| Journal | Int J Mol Med | Volume | 39 |
| Issue | 6 | Pages | 1357-1370 |
| PubMed ID | 28440464 | Mgi Jnum | J:328068 |
| Mgi Id | MGI:6883086 | Doi | 10.3892/ijmm.2017.2952 |
| Citation | Murphy S, et al. (2017) Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy. Int J Mol Med 39(6):1357-1370 |
| abstractText | X-linked muscular dystrophy is caused by primary abnormalities in the Dmd gene and is characterized by the almost complete loss of the membrane cytoskeletal protein dystrophin, which triggers sarcolemmal instability, abnormal calcium homeostasis, increased proteolysis and impaired excitationcontraction coupling. In addition to progressive necrosis, crucial secondary pathologies are represented by myofibrosis and the invasion of immune cells in damaged muscle fibres. In order to determine whether these substantial changes within the skeletal musculature are reflected by an altered rate of protein release into the circulatory system or other plasma fluctuations, we used labelfree mass spectrometry to characterize serum from the mdx4cv model of Duchenne muscular dystrophy. Comparative proteomics revealed a large number of increased vs. decreased protein species in mdx4cv serum. A serum component with greatly elevated levels was identified as the inflammationinducible plasma marker haptoglobin. This acute phase response protein is usually secreted in relation to tissue damage and sterile inflammation. Both immunoblot analyses and enzymelinked immunosorbent assays confirmed the increased concentration of haptoglobin in crude mdx4cv serum. This suggests that haptoglobin, in conjunction with other altered serum proteins, represents a novel diagnostic, prognostic and/or therapymonitoring biomarker candidate to evaluate the inflammatory response in the mdx4cv animal model of dystrophinopathy. |
