| First Author | Kochubey O | Year | 2011 |
| Journal | Neuron | Volume | 69 |
| Issue | 4 | Pages | 736-48 |
| PubMed ID | 21338883 | Mgi Jnum | J:174744 |
| Mgi Id | MGI:5140687 | Doi | 10.1016/j.neuron.2011.01.013 |
| Citation | Kochubey O, et al. (2011) Synaptotagmin increases the dynamic range of synapses by driving Ca(2)+-evoked release and by clamping a near-linear remaining Ca(2)+ sensor. Neuron 69(4):736-48 |
| abstractText | Ca(2)+-evoked transmitter release shows a high dynamic range over spontaneous release. We investigated the role of the Ca(2)+ sensor protein, Synaptotagmin2 (Syt2), in both spontaneous and Ca(2)+-evoked release under direct control of presynaptic [Ca(2)+](i), using an in vivo rescue approach at the calyx of Held. Re-expression of Syt2 rescued the highly Ca(2)+ cooperative release and suppressed the elevated spontaneous release seen in Syt2 KO synapses. This latter release clamping function was partially mediated by the poly-lysine motif of the CB domain. Using an aspartate mutation in the CB domain (D364N) in which Ca(2)+ triggering was abolished but release clamping remained intact, we show that Syt2 strongly suppresses the action of another, near-linear Ca(2)+ sensor that mediates release over a wide range of [Ca(2)+](i). Thus, Syt2 increases the dynamic range of synapses by driving release with a high Ca(2)+ cooperativity, as well as by suppressing a remaining, near-linear Ca(2)+ sensor. |
