| Primary Identifier | MGI:5804170 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Mlh1 |
| Strain of Origin | FVB/NJ x B6(Cg)-Tyr<c-2J>/J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using an sgRNA (targeting CGCTTACCAGATGGTCCGTA) and ssODN template (CACGACAGGGGTGGCTTCCTCATCCACTAGTGGAAGTGGCGACAAGGTCTACGCTTACCAGATGGATCGTACGGACTCCCGGGAGCAGAAGCTTGACGCCTTTCTGCAGCCTGTAAGCAGCCTTGGG) with CRISPR/Cas9 technology, valine codon 384 (GTC) was changed to aspartic acid (GAT) (c.1151_1152delTCinsAT, p.V384D). This mutation mimics human SNP rs63750447. |
