| First Author | Ishimura-Oka K | Year | 1995 |
| Journal | Genomics | Volume | 29 |
| Issue | 3 | Pages | 751-4 |
| PubMed ID | 8575769 | Mgi Jnum | J:29368 |
| Mgi Id | MGI:76893 | Doi | 10.1006/geno.1995.9942 |
| Citation | Ishimura-Oka K, et al. (1995) Partial structure of the mouse glucokinase gene. Genomics 29(3):751-4 |
| abstractText | A complementary DNA for glucokinase (GK) was cloned from mouse liver total RNA by a combination of the polymerase chain reaction (PCR) and mouse liver cDNA library screening. Liver- and beta-cell-specific exons 1 were isolated by PCR using mouse and rat genomic DNAs. These clones were then used to screen a mouse genomic library; three genomic clones were isolated and characterized. The mouse GK gene spans over 20 kb, containing 11 exons including a liver- or beta-cell-specific exon 1, which encodes a tissue-specific 15-aa peptide at the N-terminus of the protein. Both types of GK contain 465 amino acid residues. The predicted amino acid sequence of mouse beta-cell-specific GK showed 98 and 96% identity to the rat and human enzymes, respectively; the corresponding values are 98 and 95%, respectively, for the liver-specific GK. Several transcription factor-binding consensus sequences are identified in the 5' flanking region of the mouse GK gene. |
