| First Author | Kawahigashi H | Year | 1998 |
| Journal | Biochim Biophys Acta | Volume | 1397 |
| Issue | 3 | Pages | 305-15 |
| PubMed ID | 9582442 | Mgi Jnum | J:48396 |
| Mgi Id | MGI:1267289 | Doi | 10.1016/s0167-4781(98)00021-9 |
| Citation | Kawahigashi H, et al. (1998) A cis-acting regulatory element that affects the alternative splicing of a muscle-specific exon in the mouse NCAM gene. Biochim Biophys Acta 1397(3):305-15 |
| abstractText | The pre-mRNA encoding the neural cell adhesion molecule (NCAM) is spliced to generate NCAM isoforms containing the muscle-specific domain (MSD) during myogenesis. Utilizing chimeric NCAM minigenes, we searched for cis-acting elements that contribute to the alternative selection of exon MSDb, one of the four exons encoding MSD, and identified an intronic cis-element located downstream of exon MSDb. The cis-element acted as a negative regulator for the selection of exon MSDb in nonmuscle fibroblasts but not in myoblasts, that are already destined to differentiate into muscle cells. The suppressive effect of this cis-element on the selection of exon MSDb was released in the process of myogenesis. When MyoD was co-expressed with a minigene containing this element in fibroblasts, the suppressive effect of the cis-element was released as the cells underwent differentiation. We propose that this cis-element contributes at least as one of the regulatory elements in the differentiation state-dependent selection of MSD exons in vivo. |
