| First Author | Levenson R | Year | 1984 |
| Journal | Proc Natl Acad Sci U S A | Volume | 81 |
| Issue | 5 | Pages | 1489-93 |
| PubMed ID | 6324196 | Mgi Jnum | J:7397 |
| Mgi Id | MGI:55867 | Doi | 10.1073/pnas.81.5.1489 |
| Citation | Levenson R, et al. (1984) Molecular cloning of the mouse ouabain-resistance gene. Proc Natl Acad Sci U S A 81(5):1489-93 |
| abstractText | DNA prepared from ouabain-resistant mouse cells was able to transform ouabain-sensitive CV-1 cells to ouabain resistance after DNA-mediated gene transfer. The murine DNA fragment responsible for ouabain resistance was detected on the background of CV-1 DNA by virtue of a repetitive DNA sequence element that reacts positively with a mouse repeat DNA clone. CV-1 DNA is nonreactive with this probe. Southern analysis of several independently derived ouabain-resistant transformants indicates that the mouse ouaR gene is located on a 6.5-kilobase EcoRI restriction fragment. The 6.5-kilobase DNA fragment was initially isolated from a lambda phage library made from a ouabain-resistant secondary transformant and subsequently was subcloned in the plasmid vector pAT153. This plasmid was able to transform wild-type CV-1 cells to ouabain resistance at a frequency of about 10 cells per ng of DNA. |
