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Publication : Identification of a murine testis complementary DNA encoding a homolog to human A-kinase anchoring protein-associated sperm protein.

First Author  Anway MD Year  2002
Journal  Biol Reprod Volume  66
Issue  6 Pages  1755-61
PubMed ID  12021058 Mgi Jnum  J:76667
Mgi Id  MGI:2179914 Doi  10.1095/biolreprod66.6.1755
Citation  Anway MD, et al. (2002) Identification of a murine testis complementary DNA encoding a homolog to human a-kinase anchoring protein-associated sperm protein. Biol Reprod 66(6):1755-61
abstractText  Using differential display reverse transcriptase-polymerase chain reaction (RT-PCR) we have cloned a cDNA that encodes a putative peptide with homology to a recently reported A-kinase anchoring protein-associated protein (ASP) in human sperm. The mouse cDNA was 864 bases in length and encoded for a putative protein of 230 amino acids that had 90% amino acid similarity with the human ASP. The N terminal amino acid sequence had 65% similarity to the rat, mouse, and human protein kinase A regulatory type II sequences. Expression of the gene encoding this ASP was specific to testicular germ cells. Northern blot analysis of testis RNA from 5-, 15-, 25-, and 40-day-old mice showed expression of the ASP gene, but similar analyses of busulfan-treated germ cell-deficient mice failed to detect its expression. In addition, Northern blot analysis did not detect expression of the ASP mRNA in cultured Sertoli cells or cultured interstitial cells. Northern blot and RT-PCR analyses did not detect the ASP mRNA in mouse spleen, brain, liver, lung, heart, kidney, skeletal muscle, ovary, or Sertoli cells. In situ hybridization analysis localized the ASP mRNA to the germ cell compartment of the seminiferous tubules in the testis.
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