| First Author | Blanchard AD | Year | 2000 |
| Journal | Eur J Immunol | Volume | 30 |
| Issue | 11 | Pages | 3100-10 |
| PubMed ID | 11093124 | Mgi Jnum | J:74953 |
| Mgi Id | MGI:2159488 | Doi | 10.1002/1521-4141(200011)30:11<3100::AID-IMMU3100>3.0.CO;2-W |
| Citation | Blanchard AD, et al. (2000) Identification and characterization of SKAT-2, a novel Th2-specific zinc finger gene. Eur J Immunol 30(11):3100-10 |
| abstractText | We have identified a novel Kruppel-type zinc finger (ZF) gene, SKAT-2, which is selectively expressed by murine Th2 cells. The protein encoded by this gene has 14 C2H2-type ZF tandemly arrayed at its C terminus and N-terminal SCAN box and KRAB domains. SKAT-2 is tissue restricted in expression at the RNA level, detectable only in brain and at low levels in kidney and spleen and few hematopoietic cell lines. By in situ hybridization, SKAT-2 expression was found to peak in antigen-stimulated CD4(+) T cells after 2-3 days of culture under Th2 but not Th1 biasing conditions. This pattern of expression closely mirrored that of GATA-3 in the same cells. In transient transfection experiments in phorbol 12-myristate 13-acetate/ionomycin-stimulated EL4 cells, SKAT-2 was found to up-regulate the activity of the IL-4 but not the IL-5 promoter, contrasting with the ability of GATA-3 to activate both promoters. This result was confirmed using clones of EL4 cells stably expressing an inducible form of SKAT-2, thus SKAT-2 is a novel Th2-specific gene that may play a role in selective regulation of cytokine genes in T cells. |
